I
I
products sercives ordering301.html ordering
 
Services
>
Sitemap


 

2011 New Product List:

*You will be auto-directed to a new website, Neuro-eStore, E-Store of Neurodigitech, which provides the easiest access to the purchase of interested products!!

 
   
 
   Service List::
NDT501: Stereology-based morphological analysis of CNS (volumetric measurement, estimation of cell populations/cell counts, density and single neuron quantitation)

Unbiased stereology has been considered by modern neuroscientists as the most preferred method to measure or quantify morphological properties of the CNS.  This is particularly important in identification of subtle yet important alterations in the morphology of the brains, based on disturbances during development or in neuropsychiatric and neurodegenerative diseases.

The core focus of NDT501 service is designed to generate an accurate bio-profiling of neuronal elements (including size, shape and number), obtained in an unbiased way using proven software packages and algorithms that include standard geometric overlays, mathematical formulae, counting rules, and systematic random sampling methods.  By implementing this method, the obtained results will be accurate, efficient and more reliable than other ad hoc quantitative analyses.

With computerized systematic random-sampling layout and stabilized stage control of spatial (x-y-z) coordinates on every serial section of Region of Interest (ROIs) via Zeiss digital microscopic platform (see NDT500), we are able to quantify properties of 3D objects from a stack of 2D serial sections through an object (such as microscopic slides of a solid specimen). Our clients will expect to receive most accurate and reliable morphometric results, namely, a “3D” spatial digital profile of ROIs with a series of digitized data, ready for subsequent digital archiving and data/quality assurance.  

NDT501 service is capable of measuring brain volume and cell population estimate based on the principle of modern stereology:

3D volumetric measurement of the hippocampus

Neurogenesis: Estimation of BrdU+ cell in dentate SGZ

3D volumetric analysis of mouse hippocampal subfields

3D distribution of BrdU+ cells in mouse dentate gyrus (*Cross-lines between dentate gyri were automatically generated by the software for maintaining stabilization of spatial coordinates)

Based on the principles of the modern stereology, NDT501 service is used to systematically quantify neuron morphology, including dendritic analysis and spine counts/density along the anterior-to-posterior axis of Region of Interest (ROI) of the CNS.


Dendrograms

Spine counts distribution

Left: 3D reconstruction of CA1 pyramidal cells of mouse brain. Right: Dendrograms of basal (red) and apical (blue) dendrites of pyramidal cells. Bottom: Total lengths of apical and basal dendritic branches.

Left: Golgi-impregnated dentate gyrus & hilus (10x). Right: Representative image of spine counts (red dots) of a dendritic segment.   Bottm: Comparison of spine density (spine # / um3) between transgenic and wildtype mice 
Based on digitized serial images and quantitative data of Golgi-impregnated neurons, 3D reconstruction will provide more detailed information of neuronal properties of CNS.



Sholl analysis

3D dendritic morphology

Frequency of intersections of basal dendrites of CA1 pyramidal cells

3D reconstructed dendritic trees & spines of a pyramidal cell in the mouse cortex (*Spines can be visualized by third-party softwares)




3D neuron modeling_Quantitative stereology

3D reconstruction of Golgi-impregnated neuron

3D model of a CA1 pyramidal cells of 90-day-old mice of Alzheimer's disease

3D reconstruction of a thick (300-um) Golgi-stained section using Zeiss Axioplan2 microscopy



For data analysis, we prefer the subject IDs to be masked or blinded.  This will avoid potential evaluator's bias during the process of analysis.  Analysis of Variance (ANOVA) test will be performed and submitted to the Sponsor, with a CD that contained representative images (a low- and high-mag images per region).
Terms and Conditions

  • For quality assurance of our service, it is recommended that you discuss with us for preferred perfusion protocol and histology and/or immunolabeling protocols.
  • It is suggested that you use Gel-coated microscopic slides for tissue mounting and 0.17um-thick coverslips.
  • A 15% of the fee will be due upon authorization of the study; and the remaining fee will be due upon delivery of study results.
  • Progress of the service is contingent upon staining quality of tissues, operated by the independent contractor.
  • Should early termination occur, Neurodigitech will prorate the cost incurred and invoice the Sponsor. The first portion of the fee is non-refundable.