Tissue cryoprotection & freezing:
Fixed tissue will be infiltrated with our unique tissue cryoprotection solution (cf. Products, Cat. #NDT302) and then rapidly frozen in isopentane precooled to -70°C. The frozen tissue will be stored at -75°C before sectioning.
Remarks:
A quotation is required before placing an order.
The investigator needs to provide fixed tissue.
Embedding tissue in paraffin:
Following a thorough wash in distilled water, fixed tissue will be dehydrated through graded ethanol, cleared in xylenes, and then impregnated and embedded in paraffin.
Remarks:
A quotation is required before placing an order.
The investigator needs to provide fixed tissue.
We use various types of paraffin with the melting point ranging from 50-56°C. The investigator may choose the type of paraffin for a particular purpose.
Embedding Tissue in Epoxy Resin:
Please contact us for more information.
Terms and Conditions
For quality assurance of our service, it is recommended that you discuss with us for preferred perfusion protocol and histology and/or immunolabeling protocols.
It is suggested that you use Gel-coated microscopic slides for tissue mounting and 0.17um-thick coverslips.
A 15% of the fee will be due upon authorization of the study; and the remaining fee will be due upon delivery of study results.
Progress of the service is contingent upon staining quality of tissues, operated by the independent contractor.
Should early termination occur, Neurodigitech will prorate the cost incurred and invoice the Sponsor. The first portion of the fee is non-refundable.